Detailseite
Projekt
Characterization of the biochemistry and physiology of hydrocarbon degradation pathways by stable isotope-based approaches
Antragsteller
Privatdozent Dr. Hans Hermann Richnow
Fachliche Zuordnung
Mikrobielle Ökologie und Angewandte Mikrobiologie
Förderung
Förderung von 2009 bis 2016
Projektkennung
Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 72004890
The main objective of our project is to elucidate the isotope fractionation pattern upon anaerobic degradation pathways for several aromatic and aliphatic hydrocarbons, continuing work started in the first phase of the SPP 1319. The biochemical mechanism of the first step of the respective degradation pathway, involving the cleavage of a carbon-hydrogen bond, will be characterized by a combined analysis of hydrogen and carbon isotope fractionation. As we concentrated in the first phase of the SPP 1319 on ethylbenzene, toluene and short-chain alkanes as target compounds, we will focus in the second phase on long-chain alkanes, polycyclic aromatic hydrocarbons and alkylated phenols. Subsequently, we try to correlate fractionation pattern and amino acid sequences of enzymes catalyzing the isotope fractionating reaction. The combined results may enable us to specifically detect and quantify the anaerobic degradation of several aliphatic and aromatic hydrocarbons in the environment. A second topic of our project is to elucidate the mechanism of benzene activation under anoxic conditions, a still open reaction, and to resolve syntrophic processes in our benzene-degrading enrichment culture by stable-isotope based approaches. Generally, we will cooperate in our project with several partners of the SPP 1319 in an interdisciplinary international network.
DFG-Verfahren
Schwerpunktprogramme
Beteiligte Person
Dr. Carsten Vogt
