Project Details
Projekt
Functional dissection of transport processes during glycopeptide antibiotic production (B02)
Subject Area
Metabolism, Biochemistry and Genetics of Microorganisms
Term
from 2019 to 2023
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 398967434
Bacterial biosynthetic gene clusters of natural compounds – like antibiotics – mostly encode exporters of the compounds produced. Amycolatopsis balhimycina, producer of the glycopeptide antibiotic balhimycin, encodes the ABC exporter Tba, which was shown to be critical for balhimycin secretion but not for self-resistance of the producer strain. Here, we want to further characterize the role of Tba in balhimycin production and assess its specificity towards glycopeptide antibiotics. We will employ an integrated approach using bacterial genetics, targeted analysis of secondary metabolites, protein localization, and protein-protein interaction analysis as well as in vitro transport assays.
DFG Programme
CRC/Transregios
Applicant Institution
Eberhard Karls Universität Tübingen
Project Head
Professor Samuel Wagner, Ph.D.
