Orthotopic T cell receptor replacement by advanced non viral cell engineering (A01)

• Subject Area: Hematology, Oncology ; Immunology ; Rheumatology
• Term: since 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 452881907

Development of engineering technologies for multi-epitope or affinity-adjusted recombinant TCR T cells with high protectivity, low risk for unrecognized epitope-escape and better functional maintenance. This will be achieved via OTR- and AI-guided identification of TCRs harboring cross-reactivities against immune-escape variants or defined other epitopes, development of in vitro/in vivo protection assays for multi-epitope reactive T cells, and investigation of dual TCRalpha chain expression for next-generation TCR engineering and differentiation-dependent expression of multi-epitope and affinity-adjusted T cells.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 338: LETSIMMUN - Lymphocyte Engineering for Therapeutic Synthetic Immunity
• Applicant Institution: Technische Universität München
• Project Head: Professor Dr. Dirk Busch