Mass spectrometry of RNA and proteins in modified RNPs (C03)

• Subject Area: Biochemistry
• Term: since 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 439669440

Covalent crosslinking of proteins to the RNA in the nucleus or the cytoplasm can inhibit transcription and translation, respectively. We have recently identified that RNA-protein crosslinks (RPCs) in the messenger RNA in the cytoplasm are resolved in a dedicated translation-coupled quality control pathway. In this project, we will establish mass spectrometry-based approaches for analysis of RPCs and associated RNA modifications. We will address which RNA modifications are present in nuclear and cytoplasmic RPCs, and define the role of identified modifications in regulating the processing of crosslinked RNA and thereby RPC resolution.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 319: RMaP: RNA Modification and Processing
• Applicant Institution: Johannes Gutenberg-Universität Mainz
• Project Heads: Professorin Dr. Petra Beli; Dr. Falk Butter, until 12/2025; Professor Dr. Mark Helm; Professor Dr. Andres Jäschke, until 12/2025