Regulation of assembly and stability of the mitochondrial complexome by N-terminal processing (B13#)

• Subject Area: Biochemistry ; Cell Biology
• Term: since 2025
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 403222702

Most mitochondrial proteins contain targeting presequences that are proteolytically cleaved after import. We have shown that in human cells more than 100 of these proteins are subsequently processed by the evolutionary conserved aminopeptidase ICP55 and that this affects stability and efficiency of the respiratory chain complexes. In this project, we will investigate how ICP55-mediated amino-terminal processing affects the assembly and function of mitochondrial protein machineries.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1381: Dynamic organization of cellular protein machineries: From biogenesis and modular assembly to function
• Applicant Institution: Albert-Ludwigs-Universität Freiburg
• Project Head: Professor Dr. Pitter Huesgen