SPP 1926:  Next Generation Optogenetics: Tool Development and Application

• Subject Area: Biology ; Chemistry ; Computer Science, Systems and Electrical Engineering ; Materials Science and Engineering ; Medicine ; Physics
• Term: from 2016 to 2024
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 273915538

This is the Renewal-Proposal for the Coordination Funds of theSPP1926 'Next Generation Optogenetics: Tool Development andApplication'. It contains a report on the current, first funding period,and the application and justification for the central coordination fundsfor the second funding period.

• DFG Programme: Priority Programmes
• International Connection: Israel, Switzerland

Projects

• Analyzing the physiological function of cAMP in primary cilia using optogenetics (Applicant Wachten, Dagmar )
• Constructing of genetically encoded light-activated K+-channels with fast gating and distinct trafficking properties (Applicant Thiel, Gerhard )
• Control over mRNA translation by light-mediated uncaging of synthetic 5΄ caps in combination with fluorescent labeling of mRNAs for in vivo applications (Applicants Raz, Erez ;  Rentmeister, Andrea )
• Coordination Funds (Applicant Gottschalk, Alexander )
• Development and Application of New Optogenetic Tools Targeted to Intracellular Compartments (Applicants Hegemann, Peter ;  Rost, Benjamin )
• Development and application of RoCK, a novel Rhodopsin Cyclase/ K+ channel-based optogenetic tool for silencing of excitable cells (Applicants Baier, Herwig ;  Hegemann, Peter ;  Schneider-Warme, Franziska ;  Seifert, Ph.D., Reinhard )
• Development of new voltage imaging tools for studying mammalian neuronal plasticity in-vivo (Applicant Prevedel, Robert )
• Engineering of Chrimson for Subcellular Optogenetic application (Applicants Elstner, Marcus ;  Hegemann, Peter ;  Rost, Benjamin )
• Going full circle - optogenetic control of Ca2+ release from and reuptake into the endoplasmic reticulum (Applicants Gottschalk, Alexander ;  Lehnart, Stephan E. ;  Sasse, Philipp )
• Optical Stimulation of the Auditory Pathway by Blue-Shifted Photoswitchable Glutamate Receptor Agonists. (Applicants Huet, Ph.D., Antoine ;  Moser, Tobias )
• Optogenetic control of site-specific proteolysis and protein stability (Applicants Essen, Lars-Oliver ;  Taxis, Christof )
• Optogenetic manipulation of cell contraction signal network dynamics in tumors (Applicant Nalbant, Ph.D., Perihan )
• Optogenetic silencing tools for precise, all-optical analysis of synaptic circuits (Applicants Soba, Peter ;  Wiegert, Jörn Simon )
• Optogenetics-assisted identification of the rapid stress receptor in zebrafish (Applicant Ryu, Ph.D., Soojin )
• Photo/photoredox-switchable ligands as chemical tools for optogenetics (Applicants Lang, Kathrin ;  Sumser, Martin ;  Thorn-Seshold, Oliver )
• Photoactivated RNA Binding in a Blue-Light Receptor Enables Optoribogenetics (Applicants Mayer, Günter ;  Möglich, Andreas )
• Photoswitchable cell-penetrating PNAs for the manipulation of quiescence during regenerative myogenesis (Applicants Kassel, Olivier ;  Vazquez, Olalla )
• Photoswitchable Lipids for the Optical Control of Mechanosensitive Ion Channels (Applicants Bondar, Ph.D., Ana Nicoleta ;  Heberle, Joachim ;  Sumser, Martin )
• Red-light-regulated actuators for spatiotemporal control of opsin expression and modulation of cell-cell interactions within the prefrontal circuit during impulse control (Applicants Diester, Ilka ;  Möglich, Andreas ;  Ruther, Patrick ;  Zurbriggen, Matias Daniel )
• Second generation genetically encoded photosensitizers and killers for systems neuroscience (Applicants Griesbeck, Oliver ;  Reiff, Dierk F. )
• Shedding light on myogenesis: using optogenetics to investigate myoblast differentiation and muscle regeneration (Applicants Kassel, Olivier ;  Di Ventura, Ph.D., Barbara )
• Shrimp Rhodopsins as new far-red absorbing optogenetic tools (Applicant Hegemann, Peter )
• Tuning wavelength and G protein specificty of Melanopsin for optogenetic control of G protein signaling pathways. (Applicants Brügmann, Tobias ;  Gerwert, Klaus ;  Herlitze, Stefan )

• Spokesperson: Professor Dr. Alexander Gottschalk