Deubiquitinating enzymes in selective autophagy (D03 (A05))

• Subject Area: Biochemistry ; Cell Biology
• Term: from 2016 to 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 259130777

Dynamic protein ubiquitination plays an essential regulatory role in selective autophagy. This project aims to provide a comprehensive view on the function of deubiquitinating enzymes (DUBs) in different selective autophagy pathways. To systematically analyze DUBs that regulate mitophagy, pexophagy, and ER-phagy, we will perform a multiplex CRISPR/Cas9-based genetic screen targeting individual human DUBs and all possible combinations of DUB pairs. To study the mechanistic and functional role of identified DUBs in selective autophagy pathways, we will employ quantitative mass spectrometry-based proteomics, imaging, and biochemical methods.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1177: Molecular and Functional Characterization of Selective Autophagy
• Applicant Institution: Goethe-Universität Frankfurt am Main
• Project Heads: Professorin Dr. Petra Beli; Dr. Anja Bremm